LUQUE GUILLERMINA LETICIA
Congresos y reuniones científicas
Título:
New Electrochemical (Bio)sensing Strategies Based on the Use of Dispersed Carbon Nanotubes
Autor/es:
G.A. RIVAS, N.F. FERREYRA, M.C. RODRÍGUEZ, M.D. RUBIANES, M.L. PEDANO, G.L. LUQUE, F.A. GUTIERREZ, P.R. DALMASSO, A. GASNIER, E.N. PRIMO, Y. JALIT, F.N. COMBA, M.V. BRACAMONTE
Reunión:
Congreso; The 14th International Meeting on Chemical Sensors; 2012
Resumen:
Multi-walled carbon nanotubes (MWCNTs) dispersed in different polyelectrolytes were used to
develop electrochemical (bio)sensors by drop-coating of glassy carbon electrodes (GCEs). MWCNTs
dispersed in polyhistidine were used for the quantification of uric acid or dopamine in the presence of
large excess of ascorbic acid, and as platform to build glucose biosensors by self-assembling of
glucose oxidase (GOx). The strong interaction of calf thymus-double stranded DNA (dsDNA) with the
walls of bamboo-MWCNTs allowed us to obtain stable dispersions and modified GCEsfor sensitive
dopamine sensing. The usefulness of GOx to disperse MWCNTs was also demonstrated. The enzyme
not only disperses MWCNTs in a very efficient way, but also gives to them excellent biorecognition
properties and allows to use it as platform for further immobilization of dsDNA. MWCNTs dispersed in
polyethylenimine allowed the development of stable and robust electrochemical sensors for dopamine
in complex mixtures and dsDNA through the surface-complex formation. In summary, the analytical
performance of the (bio)sensors obtained by modification with CNT dispersions are the result of the
combination of the efficiency in the dispersion of MWCNTs and the properties that the dispersing
agent provide to them.
develop electrochemical (bio)sensors by drop-coating of glassy carbon electrodes (GCEs). MWCNTs
dispersed in polyhistidine were used for the quantification of uric acid or dopamine in the presence of
large excess of ascorbic acid, and as platform to build glucose biosensors by self-assembling of
glucose oxidase (GOx). The strong interaction of calf thymus-double stranded DNA (dsDNA) with the
walls of bamboo-MWCNTs allowed us to obtain stable dispersions and modified GCEsfor sensitive
dopamine sensing. The usefulness of GOx to disperse MWCNTs was also demonstrated. The enzyme
not only disperses MWCNTs in a very efficient way, but also gives to them excellent biorecognition
properties and allows to use it as platform for further immobilization of dsDNA. MWCNTs dispersed in
polyethylenimine allowed the development of stable and robust electrochemical sensors for dopamine
in complex mixtures and dsDNA through the surface-complex formation. In summary, the analytical
performance of the (bio)sensors obtained by modification with CNT dispersions are the result of the
combination of the efficiency in the dispersion of MWCNTs and the properties that the dispersing
agent provide to them.
