Anticandidal activity of Annona emarginata (SCHLTDL.) H. RAINER

DOLAB, JUAN G; LIMA, BEATRIZ; HERRERA CANO, NATIVIDAD; GOMEZ, J.; CABALLERO, D.; PETENATTI, ELISA; TAPIA, ALEJANDRO; ENRIZ, R.

Mendoza

Congreso; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2018

The infusions and decoctions of the leaves of Annona emarginata (Schltdl.) H. Rainer (=Rollinia emarginata Schltdl.) are used infolk medicine as a gargle against throat pain orfor calming the toothache by absorbing juice produced by chewing the leaves. Theanticandidal properties of extracts from Annona emarginata flowers, fruits, leaves, stem barks were tested against an extended panelof standardized and clinical isolated of Candida albicans and Candida tropicalis respectively, according to the guidelines of theClinical and Laboratory Standards Institute (CLSI). The fruits global methanolic extract (FGME) showed a very strong antifungalactivity against C. albicans ATCC 10231 and clinical isolated (MICs 62.5-100 g/mL).The bioassay-guided fractionation bySephadex LH 20 and silica gel column of the FGME extracts led to the isolation of compound(R)-2-(4-methylcyclohex-3-en-1-yl)propan-2-yl (E)-3-(4-hydroxyphenyl)acrylate(1), which showed a strong anticandidal activity (MIC =6.25 µg/mL) against C.albicans ATCC 10231.The main antifungal isolated compound was chemically elucidated using 1D and 2D NMR, IR, UV, HRMSexperiments and previously published date.Authors are grateful to CICITCA-UNSJ, UNSL and UNC