Heterochromatin and rDNA patterns in Solanum species of the Morelloid and Dulcamaroid clades (Solanaceae)

MOYETTA, NATALIA R.; URDAMPILLETA, JUAN D.; CHIARINI, FRANCO E.; BERNARDELLO G.

PLANT BIOSYSTEMS

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2016

1126-3504

he heterochromatin distribution and the position of 18-5.8-26S and 5S rDNA lociwere determined in 13 species of Solanum of the Morelloid and Dulcamaroid clades. The CMA/DAPI staining and FISH was employed. Two types of constitutive heterochromatinwere determined: CMA+/DAPI- associated to NOR and CMA+/DAPI- distributed as terminalbands. In the Morelloid clade, CMA+/DAPI- bands were found in five species, while in the Dulcamaroid clade, only S. angustifidum presented this feature. In the Morelloid clade, from 2 to 4 18-5.8-26S rDNA loci occupied terminal positions, and 2 rDNA 5S loci were found withvariable positions (terminal, intercalary and centromeric). In the Dulcamaroid clade, were detected 2 terminal 18-5.8-26S rDNA loci, with the exception of S. salicifolium which possessed 4 such loci, and 2 to 4 5S rDNA loci. Solanum crispum is the only species possessing the 5S in synteny with 18-5.8-26S rDNA loci. Karyotype features, chromosome banding pattern as well as the location of ribo