A novel method to prepare highly enriched primary cultures of chicken retinal horizontal cells

LUIS P. MORERA ; NICOLAS M. DIAZ; MARIO E. GUIDO

ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2012 vol. 101 p. 44 - 44

p style="TEXT-ALIGN: justify; LINE-HEIGHT: 200%; MARGIN: 0cm 0cm 0pt" class="MsoNormal">The retina is a heterogeneous tissue composed of different neuronal cells (photoreceptor (PRC), horizontal (HC), amacrine, bipolar and retinal ganglion RGC cells) and glial cells. Our aim in this work was to purify HCs from the chicken embryonic retina to obtain primary cultures highly enriched in these cells for further characterization. To this end, disaggregated retinas of chicken embryos at day 15 were subjected to a discontinuous bovine serum albumin (BSA) gradient of concentrations raging from 1 to 4%. After centrifugation, cells collected from the different phases were cultured for 4 days and characterized by immunochemistry and cell morphology. Phases were examined&n