DIFFERENTIAL RESPONSES OF THE MAMMALIAN RETINAL GANGLION CELL LINE RGC-5 TO PHYSIOLOGICAL STIMULI AND TROPHIC FACTORS

PAULA S. NIETO; VICTORIA A. ACOSTA-RODRÍGUEZ; DIEGO J. VALDEZ; MARIO E. GUIDO

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2010 vol. 57 p. 216 - 216

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt">The rat retinal ganglion cell (RGC) line RGC-5 constitutes a widely used model for studying physiological processes in retinal cells. In this paper we investigated the expression of clock and immediately early genes, and calcium mediated responses to physiological stimuli in differentiated and mitotically active RGC-5 cells. To this end, we attempted to differentiate the RGC-5 cells with a variety of effectors classically used to induce morphological differentiation. No sign of morphological differentiation was observed after 24 h of treatment with BDNF (80 ng/mL), NGF (100 ng/mL) and retinoic acid (20 ng/mL), among others. Only staurosporine (SSP) was able to promote neurite outgrowth at concentrations ranging from 53.5 to 214 nM. However, apop