The organophosphate chlorpyrifos disturbs redox balance and triggers antioxidant defense mechanisms in JEG-3 cells

CHIAPELLA, GRACIELA; FLORES-MARTÍN, JESICA; RIDANO, MAGALI EVELIN; REYNA, LUCIANA; MAGNARELLI DE POTAS, GLADYS; PANZETTA-DUTARI, GRACIELA MARÍA; GENTI-RAIMONDI, SUSANA

W B SAUNDERS CO LTD

Lugar: Londres; Año: 2013

ntroduction: Reactive oxygen species (ROS) are produced by a number of physiological and pathological processes which influence the function of a diverse array of cellular events. An imbalance between ROS generation and elimination was reported for different environmental xenobiotics exposure. Here, we analyzed the effect of chlorpyrifos (CPF) on the JEG-3 cell antioxidant defense in conditions where cell viability and morphology were not altered. Methods: Acetylcholinesterase (AChE) activity, reduced glutathione (GSH) content and catalase (CAT)antioxidant enzyme activity were measured by biochemical studies. ROS production was detected using the fluorogenic probe 20,70-dichlorodihydrofluorescein diacetate. The transcript level of superoxide dismutase enzyme 1 (SOD1), glutathione reductase (GR), heme oxygenase-1 (HO-1), and nuclear factor E2-related factor 2 (Nrf2) as well as Nrf2 protein amount were analyzed by quantitative real time PCR and Western blot, respectiv