Effects of rapamycin in combination with fludarabine on primary chronic lymphocytic leukemia cells

RODRIGUEZ, CECILIA M.; BUSSI, CLAUDIO; ARROYO, DANIELA S.; SASTRE, DARIO; HELLER, VIVIANA; STANGANELLI, CARMEN; SLAVUTSKY, IRMA; IRIBARREN, PABLO

LEUKEMIA AND LYMPHOMA

TAYLOR & FRANCIS LTD

Año: 2019 vol. 60 p. 1299 - 1299

1042-8194

hronic lymphocytic leukemia (CLL) is the most commontype of leukemia in adults, characterized by the expansionof a population of monoclonal CD5þB-lymphocytesthat accumulate in the blood, bone marrow, and secondarylymphoid tissues [1]. CLL continues to be an incurabledisease and can arise in two forms, aggressive and indolent,both characterized by a typical defect in apoptosis.Unfavorable prognosis is associated with the absence ofmutations in the immunoglobulin heavy chain variable(UM-IGHV) genes and by the expression of CD38 andhigh level of 70 kD zeta-associated protein (ZAP-70),among other markers [2,3]. Chromosomal alterations aredetected in >80% of cases and can discriminate patientswith different outcomes [4]. Deletions at chromosome13q14 are the most frequent genomic aberrationdetected by interphase fluorescence in situ hybridization(FISH) analysis. Interestingly, the microRNA miR-15a andmiR-16-1, which target the antiapoptotic molecule Bcl-2,are located in this chromosomal reg