Relevance of protein–protein interactions on the biological identity of nanoparticles

VASTI, CECILIA; BONNET, LAURA V.; GALIANO, MAURICIO R.; ROJAS, RICARDO; GIACOMELLI, CARLA E.

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Año: 2018 vol. 166 p. 330 - 330

0927-7765

onsidering that the use of nanoparticles (NPs) as carriers of therapeutic or theranostic agents hasincreased in the last years, it is mandatory to understand the interaction between NPs and living systems.In contact with biological fluids, the NPs (synthetic identity) are covered with biomolecules that form aprotein corona, which defines the biological identity. It is well known that the protein corona formationis mediated by non-specific physical interactions, but protein?protein interactions (PPI), involving spe-cific recognition sites of the polypeptides, are also involved. This work explores the relationship betweenthe synthetic and biological identities of layered double hydroxides nanoparticles (LDH-NPs) and theeffect of the protein corona on the cellular response. With such a purpose, the synthetic identity wasmodified by coating LDH-NPs with either a single protein or a complex mixture of them, followed bythe characterization of the protein corona formed in a commonly used cel