Magnetically Controlled Insertion of Magnetic Nanoparticles and Magnetic Nanoparticles@drugs into Membrane Model.

SARA NATALIA MOYA BETANCOURT; CANDELARIA I. CÁMARA; JUAREZ A. VALERIA; JULIETA S. RIVA

Córdoba

Congreso; Encuentro Anual SAB; 2023

Argentine Society of Biophysics (SAB)

Superparamagnetic iron oxide nanoparticles (SPIONs) coated with polysaccharides show potential applications in many biomedical fields. Due to this magnetic response, SPIONS are attracted to high magnetic flux density and can displacement in the direction of the field. SPIONs have now gained much importance due to their potential application as magnetic material and magnetic manipulation capabilities. Despite all the potential applications in the biomedical field, there is not so much information on how the presence of a magnetic field affects the incorporation into membrane models. With the aim of studying the effect of a magnetic field on the insertion of SPIONS coated with chitosan or diethylaminoethyl dextran into phospholipid monolayers, a magnetic field of 6.3 mT was applied. Distearoyl phosphatidic acid (DSPA) and distearoyl phosphatidylethanolamine (DSPE) were used to form phospholipid monolayers.We have found that the driving force of the SPIONs insertion into the monolayer is mostly determined by electrostatic interaction. The results indicated that the a magnetic field induces an enhancement of the insertion of the SPIONs into DSPA preformed monolayer, demonstrating that a previous electrostatic interaction between MNPs and phospholipid preformed monolayer needs to take place to enhance the incorporation process. These interactions between SPIONs and DSPE are less strongly due to the zwitterionic nature of this phospholipid. Besides, Insertion experiments demonstrate that diclofenac (DCFN) is not absorbed into DSPA monolayer. The insertion of composites SPIONs:DCFN has larger MIP (maximum insertion pressure) values, indicating that the SPIONs are adsorbed on the monolayer with the drugs. The presence of the magnetic field favors the incorporation of SPIONs:DCFN, obtaining very significant increases in the values of MIP. The fact that the composite SPIONs:DCFN present greater MIP values indicates that SPIONs can act as good drug delivery systems.