Antioxidant status in rabbit aqueous humor after instillation of ascorbyl laurate-based nanostructures

NATALIA ANGEL VILLEGAS;; LUIS IGNACIO TARTARA; GABRIELA CABALLERO; VILMA CAMPANA; DANIEL ALBERTO ALLEMANDI; SANTIAGO DANIEL PALMA; DANIEL ALBERTO ALLEMANDI; SANTIAGO DANIEL PALMA

PHARMACOLOGICAL REPORTS

POLISH ACAD SCIENCES INST PHARMACOLOGY

Lugar: Netherlands; Año: 2019

1734-1140

ackgroundThe aim of this work was evaluate the antioxidant effect of ascorbyl laurate (ASC12) based nanostructures applied topically to the cornea of ocular normotensive and hypertensive rabbits. The ASC12 was chosen for its capacity to form liquid lyotropic crystal and keeps its free radical trapping power.MethodsThe hypertension model was performed in six rabbits and was obtained by the application of intracameral injections of alpha-chymotrypsin in the right eye. A single 50 ml dose of ascorbyl laurate coagel 2% w/v (COA-ASC12) was applied topically to the cornea of six normotensive and six hypertensive rabbits. The aqueous humor samples were obtained before and after instillation of COA-ASC12 at different times (2 h and 4 h). Antioxidant capacity was determined via the reduction reaction with iron and tripyridyltriazine (FRAP) and the total proteins were measured using the Bradford reagent.ResultsThe kinetic antioxidant capacity in the aqueous humor of normotensi