DESIGN AND ANALYSIS OF AN EFFICIENT GLAUCOMA MODEL FOR EVALUATION OF PHARMACOLOGICAL TRIALS

GABRIELA AYLÉN CABALLERO; NATALIA ANGEL VILLEGAS; DAVID CÉSAR CREMONEZZI; VILMA RENEÉ CAMPANA; SANTIAGO DANIEL PALMA2; DANIEL ALLEMANDI; LUIS IGNACIO TÁRTARA

Explor Anim Med Res

-

Año: 2022

2277-470X

BSTRACT: Glaucoma is a multifactorial progressive optic neuropathy whose main risk factor is intraocular hypertension(IOH). It generates loss of nerves and is the primary cause of irreversible blindness worldwide. The objective of this workwas to develop a glaucoma model in rabbits and analyze the anatomical, functional and biochemical changes over timethrough intraocular pressure (IOP), electroretinography (ERG), antioxidant capacity with FRAP essay. in aqueous humor(AH), and histopathology with quantification of retinal ganglion cells (RGC). 24 female New Zealand white rabbits wereused. In 12 animals, glaucoma was induced by injection of µ-chymotrypsin. During the postoperative period, the treatmentand control groups were examined weekly. 7 days after surgery, IOP (mmHg) was 18.30 ± 1.75 in the treatment group and13.59 ± 0.63 in the control (p