Bacterial biofilm evidence in nasal polyposis

MARIO E. ZERNOTTI; NATALIA ANGEL VILLEGAS; MAGDALENA ROQUES REVOL; CARLOS E. BAENA-CAGNANI; JULIO E. ARCE MIRANDA; MARISA E. PAREDES; INÉS ALBESA; MARÍA G. PARAJE. (MARIO E. ZERNOTTI Y NATALIA ANGEL VILLEGAS CONTRIBUYERON DE IGUAL MANERA EN EL TRABAJO)

ESMON PUBLICIDAD S A

Lugar: España; Año: 2009 vol. 20 p. 1 - 1

ntroduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have beenimplicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. Wepropose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities as biofi lms.Objective: To determine and characterize the presence of biofi lms in CRS/NP.Methods: We performed a prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. Ten patients withoutCRS/NP who underwent septoplasty were included as a control group. Tissue samples were obtained from the inferior turbinate mucosae.The bacteria were isolated and typifi ed and the material was examined in vitro using a spectrophotometer, and in vivo using opticalmicroscopy and confocal scanning laser microscopy.Results: Moderate to high in vitro biofi lm-forming capacity was