Linking cypermethrin and chlorpyrifos residues with toxicological effects on native fish Jenynsia multidentata: a laboratory study

BONANSEA ROCÍO INÉS; WUNDERLIN DANIEL ALBERTO; AMÉ MARÍA VALERIA

Buenos Aires

Congreso; 11th Congreso Latinoamericano de la Sociedad de Toxicología y Química Ambiental (SETAC),; 2015

Sociedad de Toxicología y Química Ambiental (SETAC)

The extensive use of the insecticides Cypermethrin (CYP) and Chlorpyrifos(CPF) in Argentina for agriculture and domestic purposes resulted in the presence of these pesticides in local rivers and the exposure of non-target organisms. Moreover, CYP and CPF residues were detected in different organs of the native fish Jenynsia multidentata exposed to these pesticides singly as well as in technical and commercial mixtures under laboratory conditions. The aim of the present study was to evaluate the toxic effects of CYP and CPF using biochemical and behavioral biomarkers when the J. multidentata was exposed to the same chemical stress. Moreover, we explore the linkage between the responses of biomarkers and the pesticides residues measured in fish tissues. Thus, adultfemale fishes were exposed over 96 h to 0.04μg/L CYP; 0.4μg/L CPF; 0.04μg/LCYP + 0.4μg/L CPF in a technical mixture; and 0.04μg/L CYP + 0.4μg/L CPFin a mixture of commercial products. Forty five variables were measured indifferent fish organs (biotransformation, antioxidant and, cholinesterase enzymes activities; P-glycoprotein and aromatase expression; oxidative damage in lipids and proteins) as well as several swimming behavior parameters. Then,exploratory multivariate analysis identified differences among treatmentsaccording to the biomarkers response. Linear discriminant analysis was furtherapplied to identify 1 or 2 biomarkers per organ that better explain the variability among treatments. Those selected biomarkers responses were included in an Integrated Biomarker Response index (IBR) which allowed to arrange thetreatments according to an increase in the effect on J. multidentata: Control