MONGI BRAGATO BETHANIA DEL CARMEN
Congresos y reuniones científicas
Título:
Enkephalinergic system is involved in cocaine induced behavioral sensitization and the associated increase in brain-derived neurotrophic factor in nucleus accumbens
Autor/es:
MONGI BRAGATO B; AVALOS MP; ZAMPONI E; MASCÓ DANIEL; ZIMMER A; CANCELA LILIANA
Lugar:
Huerta Grande
Reunión:
Congreso; XXVIII Annual Congress of the Argentine Society of Neurosciences (SAN); 2013
Institución organizadora:
SAN
Resumen:
Thebehavioral sensitization involves a complex interplay between (da) and othersas glutamate (glu), opioid peptides and brain-derived neurotrophic factor growth (bdnf).
bdnfregulate drug-induced long-term neuroadaptations that encompass alterations inmolecular components at the synapse and changes in gene expression. bdnf is reported to alter glu and datransmission in the brain areas associated with cocaine-induced sensitization. themain goal of this study was to demonstrate the involvement of theenkephalinergic (enk) system in cocaine-induced behavioral sensitization, and theirassociation with changes in bdnf and its receptor. male c57b/6j wild type (wt)and preproenk knockout (ko penk) mice were daily treated with cocaine (15mg/kgi.p.) and vehicle for 9 days. on day 21 of the treatment the followingexperiments were done: 1) immunofluorescence: bdnf and trkb levels weredetermined in pfc, nac, dorsal striatum and vta in response to saline andcocaine challenge. 2) western blot: for ptrkb levels in these brain areas. we found that chronic cocaine administrationincreases bdnf and ptrkb levels in nac and vta compared to controls animals.these effects are absent in ko mice penk (-/-).these results demonstrate thatenk system is involved in cocaine- induced alterations in neurotrophic factorsin the nac and indicate thatpreproenk-derived opioid peptides arestrongly involved in the long-term plastic changes underlying behavioralsensitization to cocaine