Resumen:
ation, aiming to unveil the chemical entity responsible for the trypanocidal activity. Besides, a revision of thephytochemical study of this species, based on previous reports of the antiparasitic psilostachyins A and C as maincompounds, was conducted. To improve the biological properties of a plant extract through a simple chemicalreaction, the oxidative diversification of the dichloromethane extract of this plant species was carried out. Abioguided fractionation of a chemically modified extract was performed by evaluating the inhibitory activityagainst Trypanosoma cruzi trypomastigotes. This experiment led to the isolation of one of the most active compounds. In general terms, epoxidized metabolites were obtained as a result of the oxidation of the majormetabolite of the species. The trypanocidal activity of some tested metabolites overperformed the reference drug,benznidazole, displaying no cytotoxicity at trypanocidal concentrations. Key structure-activity relationshipswere obtain