CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells

GARCÍA, IRIS A.; DEMICHELIS, VANINA TORRES; VIALE, DIEGO L.; DI GIUSTO, PABLO; EZHOVA, YULIA; POLISHCHUK, ROMAN S.; SAMPIERI, LUCIANA; MARTINEZ, HERNÁN; SZTUL, ELIZABETH; ALVAREZ, CECILIA

JOURNAL OF CELL SCIENCE

COMPANY OF BIOLOGISTS LTD

Año: 2017 vol. 130 p. 4155 - 4155

0021-9533

any secretory cells increase the synthesis and secretion of cargo proteins in response to specific stimuli. How cells couple increased cargo load with a coordinate rise in secretory capacity to ensure efficient transport is not well understood. We used thyroid cells stimulated with thyrotropin (TSH) to demonstrate a coordinate increase in the production of thyroid-specific cargo proteins and ER-Golgi transport factors, and a parallel expansion of the Golgi complex. TSH also increased expression of the CREB3L1 transcription factor, which alone caused amplified transport factor levels and Golgi enlargement. Furthermore, CREB3L1 potentiated the TSH-induced increase in Golgi volume. A dominant-negative CREB3L1 construct hampered the ability of TSH to induce Golgi expansion, implying that this transcription factor contributes to Golgi expansion. Our findings support a model in which CREB3L1 acts as a downstream effector of TSH to regulate the expression of cargo proteins, and simultaneousl