Congresos y reuniones científicas
Título:
¨Molecular and cellular response of human trophoblast cells exposed to chlorpyrifos¨
Autor/es:
RIDANO ME; RACCA AC; GENTI-RAIMONDI S; PANZETTA-DUTARI GM
Reunión:
Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2012
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Chlorpyrifos (CPF) is one of the most widely used pesticides.
Several reports suggest that prenatal exposure leads to cognitive
impairments and increased risk of pregnancy disorders. Although
placental function and differentiation is intimately related to fetal
health, information on CPF´s effect on placenta is still scarce.
Previously, we reported that CPF increased the expression of the
ABCG2 efflux transporter and ßhCG subunit in JEG-3
choriocarcinoma cell line. Herein, we assessed primary human
cytotrophoblasts (CTBs) response to CPF exposure. CTBs purified
from normal term placentas were exposed to 0-100μMCPF during
22 or 64h. Under control conditions, CTBs do not proliferate but
differentiate and fuse forming syncytium-like structures. In the
presence of CPF, no morphological signs of cell damage or
impairment of syncytialization were detected, while mitochondrial
metabolic rate, measured using MTT, was augmented. qRT-PCR
revealed an early (22h) increase in ßhCG and p-glycoprotein efflux
transporter and a late (64h) increase inABCG2 expression in CPFtreated
CTBs. Instead, mRNA level of the PSG3 differentiation
marker and KLF6 transcription factor remained unmodified at the
time points assayed. These results suggest that villous trophoblasts
may activate protection mechanisms in order to preserve placental
function.