Antinociceptive effect and identification of berberine alkaloid in Berberis ruscifolia extracts

DEL GAUDIO, MICAELA PAULA; KRAUS, SCHEILA IRIA; MELZER, TAYZA MARTINS; BUSTOS, PAMELA SOLEDAD; ORTEGA, MARÍA GABRIELA

JOURNAL OF ETHNOPHARMACOLOGY

ELSEVIER IRELAND LTD

Año: 2023 vol. 305

0378-8741

thnopharmacological relevance: Aerial parts (leaves and stems) of Berberis ruscifolia Lam. are a usual preparation as an analgesic, anti-inflammatory, antimalarial, antibacterial, and digestive in folk medicine. However, there were no previous studies of its chemical composition and biological activity related to analgesic effects. The objective of the study: The evaluation of the anti-nociception of the infusion (I), the decoction (D), and the ethanolic extract (EE) obtained from aerial parts of B. ruscifolia and its main chemical constituent in them, in mouse models. Material and methods: The chemical constituent of B. ruscifolia extracts was evaluated and quantified by LC-MS and HPLC methodology. The inhibition of nociception in mice was analyzed by formalin and acetic acid-induced contortions tests. Also, when the formalin test was performed to evaluate the antinociceptive activity, the inhibition of edema formation and the antipyretic effect of each extract were simultaneously ev