Saturated fatty acid toxicity in Caenorhabditis elegans: an approach to studying high-fat diet

MORAN, YANINA SOLEDAD; LUGO, BELÉN CATALINA; COTELO, CAMILO; ASIS, RAMÓN

Valparaíso

Congreso; Third Latin American Worm Meeting; 2023

The western diet is characterized principally by its high content of saturated fat, this and other habits unchain diseases of which of those that stand out of frequency the vascular and metabolic diseases. Stearic acid (SA) is one of these diets' most representative fatty acids. This study aimed to evaluate the effect of increased SA intake on the life cycle and lipid metabolism in C. elegans; and the chlorogenic acid (CGA, food antioxidant) ability to counteract the SA effects. We found that SA reduces the body size, reproduction, fat storage, and fat content in the N2 strain, while CGA reverts these effects. Genes involved in these mechanisms were evaluated with mutants and RNAi studies. Transcriptor factors such us hif-1, sbp-1, and the lipids metabolism enzymes atgl-1 and hosl-1 were the most prominent.