Glutamine protects intestinal calcium absorption against oxidative stress and apoptosis

MOINE, LUCIANA; DIAZ DE BARBOZA, GABRIELA EDITH; PEREZ, ADRIANA; BENEDETTO, MARIA MERCEDES; TOLOSA DE TALAMONI, NORI GRACIELA

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2017

1095-6433

he aim of this study was to investigate whether glutamine (GLN) could block the inhibition of the intestinal Ca2+ absorption caused by menadione (MEN), and elucidate the underlying mechanisms. To do this, one-month old chicks were divided in four groups: 1) controls, 2) MEN treated, 3) GLN treated and 4) GLN treated before or after MEN treatment. Intestinal Ca2+ absorption as well as protein expression of molecules involved in the transcellular Ca2+ pathway were determined. Glutathione (GSH) and superoxide anion and activity of enzymes of the antioxidant system were evaluated. Apoptosis was measured by the TUNEL technique, the expression of FAS and FASL and the caspase-3 activity. A previous dose of 0.5 g GLN/kg of b.w. was necessary to show its protector effect and a dose of 1 g/kg of b.w. could restore the intestinal Ca2+ absorption after MEN treatment. GLN alone did not modify the protein expression of calbindin D28k and plasma membrane Ca2+-ATPase, but blocked the inhibitory effec