Study on visual and non-visual opsins in a model of retinal degeneration caused by led lights.

BENEDETTO, MARIA MERCEDES; QUINTEROS QUINTANA, MARIA LUZ; GUIDO, MARIO EDUARDO; CONTIN, MARIA ANA

BIOCELL

BIOCELL

Año: 2016

0327 9545

he retina is a complex neural circuit that transduces light into a pattern of electrical Impulses to the brain. This tissue fulfils a visualrole through rods and cones and the synchronization of circadian rhythms by a subpopulation of photosensitive ganglion cells (GC).The process of retinal degeneration (RD) by low intensity LED light exposure is not well characterized. Previously, we demonstratedrods and cones death and a reduced functionality of these cells after 4 days of light exposure.The goal of this work was to evaluate theconstant low LED light stimuli effects over visual photoreceptors and GC, studying the oxidative stress mechanism, Rhodopsin (Rho),Opn4 and Opn5 expression and Rho phosphorylation. Male Albino Wistar rats were exposed to white LED light (200 lx) for 1 to 8days. We found a significant increase in retinal reactive oxygen species production after 5 days of light. The number of GC was notaltered, however, the expression of Opn4 increased and its location varied