BENEDETTO MARIA MERCEDES
Congresos y reuniones científicas
Título:
GLUTAMINE PREVENTS INTESTINAL CALCIUM ABSORPTION INHIBITION TRIGGERED BY MENADIONE MAINTAINING THE REDOX STATE
Autor/es:
BENEDETTO M; DÍAZ DE BARBOZA G; TOLOSA DE TALAMONI N
Lugar:
San Juan
Reunión:
Congreso; Segunda Reunión Conjunta de Sociedades de Biología de Córdoba; 2011
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
Glutamine (GLN) is an aminoacid with restorative properties of
the redox state in tissues exposed to oxidant drugs. We have demonstrated
that menadione (MEN) decreases intestinal Ca absorption
triggering oxidative stress and apoptotic death of enterocytes.
In this work we explored whether GLN could prevent the inhibitory
effect caused by MEN on the Ca absorption. Chicks (4 weeks
old Cobb-Harding) were treated with 1g GLN/kg bw per os and
with 2.5μmol MEN /kg bw intraperitoneally (60 or 90 min later).
Both drugs acted together for 30 min. Controls were treated with
vehicle. We measured intestinal Ca absorption by “in situ” ligated
loop technique. The activities of enzymes superoxide dismutase
(SOD) and catalase (CAT) and total glutathione content (GSH) were
determined by spectrophotometry. GLN administration 60 or 90min
before MEN injection prevented the fall in intestinal Ca absorption
triggered by MEN. GLN increased GSH levels, restoring the control
values. GLN avoided the induction of SOD and CAT activities
by MEN. These data suggests that GLN is capable to prevent the
decrease in the intestinal Ca absorption caused by MEN, probably
by the restoration of control values of the GSH content and the
activities of SOD and CAT.
the redox state in tissues exposed to oxidant drugs. We have demonstrated
that menadione (MEN) decreases intestinal Ca absorption
triggering oxidative stress and apoptotic death of enterocytes.
In this work we explored whether GLN could prevent the inhibitory
effect caused by MEN on the Ca absorption. Chicks (4 weeks
old Cobb-Harding) were treated with 1g GLN/kg bw per os and
with 2.5μmol MEN /kg bw intraperitoneally (60 or 90 min later).
Both drugs acted together for 30 min. Controls were treated with
vehicle. We measured intestinal Ca absorption by “in situ” ligated
loop technique. The activities of enzymes superoxide dismutase
(SOD) and catalase (CAT) and total glutathione content (GSH) were
determined by spectrophotometry. GLN administration 60 or 90min
before MEN injection prevented the fall in intestinal Ca absorption
triggered by MEN. GLN increased GSH levels, restoring the control
values. GLN avoided the induction of SOD and CAT activities
by MEN. These data suggests that GLN is capable to prevent the
decrease in the intestinal Ca absorption caused by MEN, probably
by the restoration of control values of the GSH content and the
activities of SOD and CAT.
