Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated Gold Nanoparticles: From One-Step Synthesis to in Vivo Cytocompatibility

SILVERO, M. JAZMÍN; ROCCA, DIAMELA M; ARTUR D LA VILLARMOIS, EMILCE; FOURNIER, KELSEY; LANTERNA, ANABEL E; PEREZ, MARIELA F; BECERRA, MARIA CECILIA; SCAIANO, JUAN C

ACS OMEGA

American chemistry Society

Año: 2018

2470-1343

hotoinduced antibacterial gold nanoparticleswere developed as an alternative for the treatment ofantibiotic-resistant bacteria. Thanks to the amoxicillin coating,they possess high in vivo stability, selectivity for the bacteria wall,a good renal clearance, and are completely nontoxic foreukaryotic cells at the bactericidal concentrations. A simpleone-step synthesis of amoxi@AuNP is described at mildtemperatures using the antibiotic as both reducing and stabilizingagent. Time-resolved fluorescence microscopy proved thesenovel nano-photosensitizers, with improved selectivity, arebactericidal but showing excellent biocompatibility towardeukaryotic cells at the same dose (1.5 μg/mL) when co-culturesare analyzed. Their stability in biological media, hemocompat-ibility, and photo-antibacterial effect against sensitive andantibiotic-resistant Staphylococcus aureus were evaluated in vitro, whereas toxicity, renal clearance, and biodistribution werestudied in vivo in male Wistar rats. The