Biopolymeric particles: Promising carriers for peptide delivery.

CHEVALIER, MERARI T; GARCÍA, MÓNICA C; GONZALEZ, DANIELA; JIMENEZ-KAIRUZ, ALVARO F; FARINA, HERNÁN; ALVAREZ, VERA

Salamanca

Congreso; Congreso Iberoamericano de Biotecnología.; 2016

Over the past decade, significant progress has been made in the development of new pharmaceutical technology platforms based on different kind of systems. In particular, polymeric microparticles are micron size entities which can be made from a wide variety of natural and synthetic polymers. Due to particle?s ability to improve the efficiency of current therapeutic treatments, this type of devices are being extensively studied and used as drug carriers and controlled release systems in the field of biomaterials, medicine and pharmacy. Considering polylactic acid capability for high loading drugs and its ability to modulate drug release, this work attempts to study the physicochemical and biopharmaceutical properties of PLA/PEG-b-PLA polylisine microparticulated carriers. Microparticles were fabricated using a proprietary electrohydrodynamic technology (Bio-Target Inc., Chigaco, IL, USA; LNK Chemsolutions LLC, Lincoln, Nebraska, USA). Briefly, for all MPs formulations, organic solutions containing all the necessary components were processed using this technology resulting in a dry collection of the specified MPs. The particles were characterized by scanning electron microscopy (SEM), thermo gravimetric analysis (TGA), differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FTIR). UV-Vis spectrophotometry was used to determine the encapsulation efficiency (EE) of the obtained system. The polylisine release from particles was also performed. Ringer solution was used as receptor media. Samples of 1.0 mL of receptor medium were withdrawn at predetermined time intervals and replaced with equal quantities of fresh medium. The amount of drug released was determined by high performance liquid chromatography (HPLC). The obtained systems showed a considerable potential for drug delivery of peptides.