The Transcription Factor NF-κB Mediates Thyrotropin-Stimulated Expression of Thyroid Differentiation Markers

GEYSELS, ROMINA CELESTE; PEYRET, VICTORIA; MARTÍN, MARIANO; NAZAR, MAGALÍ; REALE, CARLA; BERNAL BARQUERO, CARLOS EDUARDO; MIRANDA, LUCAS; MARTÍ, MARCELO ADRIÁN; VITO, PASQUALE; MASINI-REPISO, ANA MARÍA; NICOLA, JUAN PABLO

THYROID

MARY ANN LIEBERT INC

Lugar: New York; Año: 2020

1050-7256

ackground: The nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) transcription factor is a key regulator of cell survival, proliferation, and gene expression. Although activation of NF-κB signaling in thyroid follicular cells after thyrotropin (TSH) receptor (TSHR) engagement has been reported, the downstream signaling leading to NF-κB activation remains unexplored. Here, we sought to elucidate the mechanisms that regulate NF-κB signaling activation in response to TSH stimulation. Methods: Fisher rat-derived thyroid cell lines and primary cultures of NF-κB essential modulator (NEMO)-deficient mice thyrocytes were used as models. Signaling pathways leading to the activation of NF-κB were investigated by using chemical inhibitors and phospho-specific antibodies. Luciferase reporter gene assays and site-directed mutagenesis were used to monitor NF-κB-dependent gene transcriptional activity and the expression of thyroid differentiati