Dectin-1 on macrophages modulates the immune response to Fasciola hepatica products through the ERK signaling pathway

GUASCONI, LORENA; BURSTEIN, VERÓNICA L.; BECCACECE, IGNACIO; MENA, CRISTIAN; CHIAPELLO, LAURA S.; MASIH, DIANA TERESA

IMMUNOBIOLOGY.

ELSEVIER GMBH

Año: 2018 vol. 223 p. 834 - 834

0171-2985

asciolosis is a zoonotic disease of increasing importance due to its worldwidedistribution and elevated economic losses. Previously, we demonstrated thatFasciola hepatica excretory?secretory products (FhESP) induceimmunomodulatory effects on peritoneal macrophages in a Dectin-1 dependentmanner.In this study, we observed that peritoneal macrophages from naive BALB/c micestimulated in vitro with FhESP presented increased expression levels ofphosphorylated extracellular-signal-regulated kinase (ERK), and this effect wasdependent on Syk, protein kinase C (PKC) and Dectin-1. In this sense, weobserved increased levels of arginase activity, IL-10 and TGF-β in macrophagesstimulated with FhESP, which were dependent on PKC and ERK. Furthermore,we observed that the increased arginase activity, as well as in TGF-β and IL-10levels, was partially dependent on IL-10 receptor signaling in macrophages thatwere pre-incubated with anti-IL10R before being stimulated with FhESP.Taken together, t