New antibiotic-anesthetic modified release films. Preclinical evaluation on deep second degree burns

SANCHEZ MARIA FLORENCIA; GUZMAN MARIA LAURA; TOLEDO, MARÍA EUGENIA; LAINO, CARLOS; SORIA, ELIO ANDRÉS; OLIVERA, MARIA EUGENIA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias; 2019

Reunión Anual de Sociedades de Biociencias

Most important burn treatment challenges are associated with control of infections and pain; and also functional and aesthetic wound healing. The aim of this study was to evaluate the wound healing efficacy in a burn rat model of a treatment with an anesthetic-antibiotic polymeric films (AAF), compared to the reference treatment. A deep second degree burn model was used in male Wistar rats (CICUAL UNLaR protocol nº 5/18). Each anesthetized animal was injured in the dorsal region with a metallic device (1 cm ø, 90±2 ºC, 30 sec). The AAF (Argentine Patent Application nº 20190102232), control film (CF) or reference cream treatments (RC: silver sulfadiazine 1.0%, lidocaine 0.67% and vitamin A 248000 UI) were applied once daily for 21 days (n=6). Control groups: untreated (UT) and not burned. Photographs and biopsies (H&E) were taken on days 0, 7, 14 and 21. Epidermal continuity and dermal organization were evaluated with scores according to Sanchez et al1.Biopsies analysis showed that epidermis closure was reached in the order AAF > CF > UT > RC. Besides, burns treated with AAF presented complete dermis organization at day 21 and histological characters similar to unburned control. These results could be related to the favorable moist environment provided by the components of AAF, that positively impacts on the tissue recovery. In contrast, burns treated with RC did not complete its regeneration at day 21 and even a regression was observed respect to day 14. Most of UT animals presented dense dermis and absence of skin annexes (day 21). These findings suggest that the use of the AAF allowed a more rapid and better quality skin regeneration process with respect to the available reference treatment.