Promoter Sequence of Shiga Toxin 2 (Stx2) Is Recognized In Vivo, Leading to Production of Biologically Active Stx2

LETICIA V. BENTANCOR; MARIA P. MEJÍAS; ALÍPIO PINTO; MARCOS F. BILEN; ROBERTO MEISS; MARIA C. RODRIGUEZ-GALÁN; NATALIA BAEZ; LUCIANO P. PEDROTTI; JORGE GOLDSTEIN; PABLO D. GHIRINGHELLI; MARINA S. PALERMO

mBio

American society for microbiology

Año: 2013 vol. 4 p. 1 - 1

2150-7511

higa toxins (Stx) are the main agent responsible for the development of hemolytic-uremic syndrome (HUS), the most severe and life-threatening systemic complication of infection with enterohemorrhagic Escherichia coli (EHEC) strains. We previously described Stx2 expression by eukaryotic cells after they were transfected in vitro with the stx2 gene cloned into a prokaryotic plasmid (pStx2). The aim of this study was to evaluate whether mammalian cells were also able to express Stx2 in vivo after pStx2 injection. Mice were inoculated by hydrodynamics-based transfection (HBT) with pStx2. We studied the survival, percentage of polymorphonuclear leukocytes in plasma, plasma urea levels, and histology of the kidneys and the brains of mice. Mice displayed a lethal dose-related response to pStx2. Stx2 mRNA was recovered from the liver, and Stx2 cytotoxic activity was observed in plasma of mice injected with pStx2. Stx2 was detected by immunofluorescence in the brains of mice inoculated