Membrane distribution of epidermal growth factor receptors in cells expressing different gangliosides

ZURITA A.R,; CRESPO P.M,; KORITSCHONER N.P,; JOSE LUIS DANIOTTI

EUROPEAN JOURNAL OF BIOCHEMISTRY

Blackwell Publishing

Lugar: Oxford; Año: 2004 vol. 271 p. 2428 - 2428

0014-2956

font face="AdvTimes" size="2"> Gangliosides have been found to reside in glycosphingolipidenriched microdomains (GEM) of the plasma membrane and to be involved in the regulation of epidermal growth factor receptor (EGFr or ErbB1) activity.To gain further insight into the mechanisms involved in EGFr modulation by gangliosides, we investigated the distribution of EGFr family members in the plasma membrane of CHO-K1 cells, which were genetically modi.ed to express di.erent ganglioside molecules or depleted of glycolipids.Our data demonstrate that at least four di.erent sets of endogenously expressed gangliosides, including GD3, did not have a signicant efect on EGFr distribution in the plasma membrane. In addition, using confocal microscopy analysis we clearly demonstrated that the EGFr co-localizes only to a minor extent with GD3.We also explored the endogenous expression, in wild-type CHO-K1 cells, of the orphan receptor ErbB2 (which is the preferred heteroassociation