Modulation of epidermal growth factor receptor phosphorylation by endogenously expressed gangliosides

ZURITA, ADOLFO R.; MACCIONI, HUGO J.F.; DANIOTTI, JOSE LUIS

BIOCHEMICAL JOURNAL

Published by Portland Press on behalf of the Biochemical Society

Lugar: London, England; Año: 2001 vol. 355 p. 465 - 465

0264-6021

he effect of changing the ganglioside composition of Chinesehamster ovary K1 cells on the function of the epidermal growthfactor receptor (EGFr) was examined by studying the signallingpathway generated after the binding of epidermal growthfactor (EGF) both in cells depleted of glycosphingolipids byinhibiting glucosylceramide synthase activity and in cell linesexpressing different gangliosides as the result of stabletransfection of appropriate ganglioside glycosyltransferases.After stimulation with EGF, cells depleted of glycolipidsshowed EGFr phosphorylation and extracellular signal-regulatedprotein kinase 2 (ERK2) activity as parental cells expressingGM3 [ganglioside nomenclature follows Svennerholm (1963)J. Neurochem. 10, 613±623] or as transfected cells expressing mostly GM2 and GD1a as the result of stable transfectionof UDP-GalNAc:LacCer/GM3/GD3 N-acetylgalactosaminyltransferase.However, cells sta