GD1a modulates GM-CSF-induced cell proliferation

ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD

Año: 2011 vol. 56 p. 600 - 600

angliosides have been extensively described to be involved in the proliferation and differentiation of variouscell types, such including hematopoietic cells. Our previous studies on murine models of stromamediatedmyelopoiesis have shown that gangliosides are required for optimal capacity of stromal cellsto support proliferation of myeloid precursor cells, being shed to the supernatant and selectively incorporatedinto myeloid cell membranes. Here we describe the effect of gangliosides on the specific granulocyte–macrophage colony-stimulating factor (GM-CSF)-induced proliferation. For that, we used themonocytic FDC-P1 cell line, which is dependent upon GM-CSF for survival and proliferation. Cells werecultured in the presence of GM-CSF and exogenous gangliosides (GM3, GD1a or GM1) or in the absenceof endogenous ganglioside synthesis by the use of a ceramide-synthase inhibitor, D-PDMP. We observedthat exogenous addition of GD1a enhance