Regulation of ganglioside composition and synthesis is different in developing chick retinal pigment epithelium and neural retina

DANIOTTI J.L.; LANDA C.A.; MACCIONI H.J.F.

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 1994 vol. 62 p. 1131 - 1131

e examined the immunocytochemical expression of GM3 and GD3 in 3-day-old chick embryo retinal pigment epithelium (RPE) and neural retina (NR). We also compared the composition of gangliosides and the activities of key ganglioside glycosyltransferases of the RPE and NR of 8-, 12-, and 15-day old embryos. The immunocytochemical studies in 3-day-old embryos showed heavy expression of GM3 and GD3 at the inner and outer layers of the optic vesicle that are the precursors of the RPE and NR, respectively. The compositional and enzymatic studies showed pronounced differences between RPE and NR of 8-day and older embryos. HPTLC showed that at 8 days the major species were GM3 and GD3 in RPE and GD3 and GT3 in NR. As development proceeded, GD3 decreased in both tissues, GM3 became the major ganglioside in RPE, and ganglio-series gangliosides (mainly GDla) became the major species in NR. At 15 days the major species were GD1 a in NR and GM3 in RPE. Enzyme determinations showed