Gangliosides of myelosupportive stroma cells are transferred to myeloid progenitors and are required for their survival and proliferation

ZIULKOSKI A.; ANDRADE C.; CRESPO P.; SISTI E.; TRINDADE V.; DANIOTTI J.L.; GUMA F.; BOROJEVIC, R.

BIOCHEMICAL JOURNAL

Portland Press

Lugar: Londres; Año: 2006 vol. 394 p. 1 - 1

0264-6021

font face="Times-Roman" size="2"> In previous studies, we have shown that the myelopoiesis dependent upon myelosupportive stroma required production of growth factors and heparan-sulphate proteoglycans, as well as generation of a negatively charged sialidase-sensitive intercellular environment between the stroma and the myeloid progenitors. In the present study,we have investigated the production, distribution and role of gangliosides in an experimental model of in vitro myelopoiesis dependent upon AFT-024 murine liver-derived stroma. We used the FDC-P1 cell line, which is dependent upon GM-CSF(granulocyte/macrophage colony-stimulating factor) for both survival and proliferation, as a reporter system to monitor bioavailability and local activity of GM-CSF. GM3 was the major ganglioside produced by stroma,