EFFECT OF ANIDULAFUNGIN AND HUMAN MONOCYTES AGAINST Candida spp. biofilm.

ICELY, PAULA ALEJANDRA; RODRIGUEZ, EMILSE; PAIVA, CLAUDIA; MIRÓ, MARÍA SOLEDAD; VIGEZZI, CECILIA; ABIEGA, CLAUDIO; CAEIRO, JUAN PABLO; RIERA, FERNANDO; SOTOMAYOR, CLAUDIA ELENA

Congreso; XIV Forum on Fungal Infection in the Clinical Practice; 2016

INTRODUCTIONCandida Candida spp bloodstream infection is a frequent form of mycosis with high mortality rates . Biofilm formation is a potent virulence factor for Candida species, as it confers significant resistance to antifungal agents and to the innate immune response. Candida albicans and Candida parapsilosis are the most prevalent species related to the biofilm mode of growth. C. parapsilosis is a frequent cause of candidaemia, particularly among neonates, in patients with vascular catheters or parenteral nutrition and in those who have received prior antifungal agents, or have undergone transplantation (1, 2).Equinocandins such as anidulafungin are new drugs that broaden the available therapeutic arsenal for invasive fungal infection treatment, that display favorable pharmacodynamic and pharmacokinetic characteristics and have an excellent toxicological profile (3). It is unknown how antifungal agents like anidulafungin interacts with human phagocytes against Candida spp biofilms and whether anidulafungin could influence the interaction between innate immune cells and biofilms.GOALS Being C .parapsilosis the second most common cause of invasive candidiasis in Córdoba behind C. albicans, the aim of our study was to evaluate the activity of anidulafungin combined with human monocytes against Candidas spp mature biofilms of both species.MATERIAL AND METHODSMaterialsThe clinical isolates were obtained from patients from ICU with Candidemia and identified by Maldi Tof (Biomerieux ) and molecular methods as C.albicans (001 and 003 isolates) and C.parapsilosis senso stricto (008, 010 011 and 017 isolates).The THP-1 monocytic cell line (ATCCTIB202; ATCC, Manassas,VA,USA) was used as human phagocyte source.The echinocandin used in this study was anidulafungin (Pfizer). Experimental Procedure and methodsDifferent inoculums of each strain were used to obtained two type of mature biofilm classified as high (HB) and low (LB) biomass in agreement with initial use of 3X106 or 1x105 blastoconidia respectively. Each concentration was placed per well in 96 well plates at 37°C ina humidified 5% CO2 incubator for 48 h. Quantitative measurement of biofilm formation (BF) was assessed by XTT reduction assay(4) performed in triplicate for all strains and the averages and standard deviations were calculated. Strains were classified as Weak (Absorbance (A) <0.310) (WBF), Moderate (0.310