Photodynamic inactivation of multiresistant bacteria (KPC) using zinc(II)phthalocyanines

MIRETTI, MARIANA; CLEMENTI, ROMINA; TEMPESTI, TOMAS C.*; BAUMGARTNER, MARÍA T.*

BIORGANIC AND MEDICINAL CHEMISTRY LETTERS

Elsevier Ltd

Lugar: Amsterdam; Año: 2017 vol. 27 p. 4341 - 4341

0960-894X

he worldwide increase in antibiotic resistance has led to search of alternatives anti-microbial therapies such as photodynamic inactivation. The aim of this paper was to evaluate the photodynamic activity in vitro of a neutral and two cationic Zn phthalocyanines. Their photokilling activity was tested on Escherichia coli ATCC 25922 and Klebsiella pneumoniae Carbapenemase (KPC)-producing. After treating bacteria with phthalocyanines, the cultures were irradiated with white light. As a result, the bacteria were inactivated in presence of cationic phthalocyanines. The photoinactivation was dependent of the irradiation time and phthalocyanine concentration. The most effective photosensitizer on KPC-producing was Zinc(II)tetramethyltetrapyridino[2,3-b:2´,3´-g:2″,3″-l:2‴,3‴-q]porphyrazinium methylsulfate (ZnTM2,3PyPz). After irradiation using the water soluble ZnTM2,3PyPz (3. μM) the viability of KPC (30. min of irradiation) and E. coli (10. min of irradiation)