Rapamycin and Resveratrol Modulate the Gliotic and Pro-Angiogenic Response in Müller Glial Cells Under Hypoxia

SUBIRADA, PAULA V.; VAGLIENTI, MARÍA V.; JORAY, MARIANA B.; PAZ, MARÍA C.; BARCELONA, PABLO F.; SÁNCHEZ, MARÍA C.

Frontiers in Cell and Developmental Biology

Frontiers Media S.A.

Año: 2022 vol. 10

ypoxia and hypoxia-reoxygenation are frequently developed through the course of manyretinal diseases of different etiologies. Müller glial cells (MGCs), together with microglia andastrocytes, participate firstly in response to the injury and later in the repair of tissuedamage. New pharmacological strategies tend to modulate MGCs ability to induceangiogenesis and gliosis in order to accelerate the recovery stage. In this article, weinvestigated the variation in autophagy flux under hypoxia during 4 h, employing both gasculture chamber (1% O2) and chemical (CoCl2) hypoxia, and also in hypoxiareoxygenation. Then, we delineated a strategy to induce autophagy with Rapamycinand Resveratrol and analysed the gliotic and pro-angiogenic response of MGCs underhypoxic conditions. Our results showed an increase in LC3B II and p62 protein levels afterboth hypoxic exposure respect to normoxia. Moreover, 1 h of reoxygenation after gashypoxia upregulated LC3B II levels respect to hypoxia although a d