Study of the LRP1/α2M system during choroidal neovascularization

TOVO, A.; SUBIRADA, PAULA V.; VAGLIENTI, MARIA V.; LUNA PINTO, JD; SÁNCHEZ, MARÍA C.; CHIABRANDO, GA.; BARCELONA, PABLO F.

Virtual

Congreso; XIII CONGRESO NACIONAL DE INVESTIGACIÓN EN VISIÓN Y OFTALMOLOGÍA; 2021

AIVO

Objectives: Age-related macular degeneration (AMD) in its Choroidal neovascularization (CNV) stage, is the leading cause of vision loss among adults. Our lab has previously demonstrated that α2M and its receptor, LRP1, participate during retinal NV. Moreover, other authors propose LRP1 as a regulator of inflammatory responses. In the present study, we characterize the neovascular process and the proinflammatory profile, emphasizing on the participation of the α2M/LRP1 system in CNV. Materials and methods: C57BL/6 adult mice were treated with four spots of laser photocoagulation per eye. The levels of α2M and LRP1 were analyze by WB. After 7 days of laser, pro-inflammatory and pro-angiogenic profile was evaluated by qPCR. In addition, on the NV area in choroid?RPE flatmounts (isolectin B4) was studied the presence of precursor endothelial cell (CD105) by IF. Also, the mononuclear phagocyte cells, (F4/80 and Iba 1), and his LRP1 expression was evaluated by confocal microscopy. Moreover, LRP1 levels on microglia were confirm by Flow cytometry. Results: We could observe change of the α2M and LRP1 protein level in CNV animals respect to control at the different time assayed. Also, high levels of both, pro-inflammatory and pro angiogenic factors, as well as an elevated number of mononuclear phagocyte cells expressing LRP1 close to the CNV area was observed in RPE-choroid 7 days post laser. Conclusions: In sum, our results showed change of α2M and LRP1 level on the CNV animals, particularly on microglia cells. Further studies are needed to determinate the role of α2M/LRP1 system on the CNV.