Tobacco hairy root´s peroxidases are rhythmically controlled by phenol exposure

SOSA-ALDERETE LUCAS; HEBE RONCHI; MONJES, NATALIA M.; ELIZABETH AGOSTINI

ENZYME AND MICROBIAL TECHNOLOGY

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2021

0141-0229

lants like almost all living organisms, have developed a biological clock or circadianclock (CC) capable of synchronizing and adjusting various metabolic and physiologicalprocesses at certain times of the day and in a period of 24 h. This endogenoustimekeeping is able to predict the environmental changes providing adaptiveadvantages against stressful conditions. Therefore, the aim of this work was to analyzethe possible link between metabolism of xenobiotic compounds (MXC) and the CC.Synchronized Nicotiana tabacum hairy roots (HRs) were used as a validated plantmodel system, and peroxidases (PODs), key enzymes of the phase I in the MCX, wereevaluated after phenol treatment. Two POD genes were selected and their temporalexpression profiles as well as the total POD activity were analyzed in order to findcircadian oscillations either under control conditions or phenol treatment. It wasdemonstrated that these PODs genes showed oscillatory expression profiles with anultradian period (perio