THE CIRCADIAN CLOCK AND PHOSPHOLIPID METABOLISM FUNCTION IN THE GLIOBLASTOMA CELL LINE T98G

SOSA-ALDERETE LUCAS; MONJES NATALIA MARIBEL; CHEVALLIER-BOUTELL IGNACIO; GUIDO MARIO EDUARDO

Congreso; SAIB-Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2014

The circadian system temporarily regulates diverse behavioral, physiological and metabolic processes and comprises central and peripheral oscillators distributed in organs, tissues and even in immortalized cell lines. The disruption of circadian clocks leads to severe metabolic disorders (hyperlipidemia, fat liver, obesity) and higher cancer risk. Our aims were to evaluate whether the immortalized human glioblastoma T98G retains the molecular clock machineryfunctional regulating temporally clock gene (Bmal1 and Per1) and clock controlled gene (CCG) (choline kinase (CK) (alpha1) expression and the metabolic labeling of total phospholipids (PLs). To this end, T98G cells were grown in 10% FBS-supplemented DMEM for 48-72 h. After synchronization with dexamethasone (100 nM), T98G cells were maintained with or without 10% FBS-DMEM in a proliferative condition (P) or a partial arrest (PA) respectively for 48 h. Results showed that Bmal1, Per1 and the CCG CKalpha1, conserved a temporal expression under both experimental conditions (P and PA) with a period ~ 24-28 h. Also, it was found that T98G cells displayed a circadian rhythmicity in the labeling of 32P-PLs with highest levels at 20 and 48 h for both culture conditions. Results strongly suggest that the tumor cells preserve the temporal control of the molecular clock and the PL biosynthesis under the experimental conditions studied.