Sec3 exocyst component knockdown inhibits axonal formation and cortical neuronal migration during brain cortex development

BUSTOS PLONKA, FLORENTYNA

JOURNAL OF NEUROCHEMISTRY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2021 vol. 160 p. 203 - 203

0022-3042

eurons are the largest known cells, with complex and highly polarized morphologiesand consist of a cell body (soma), several dendrites, and a single axon. The establishmentof polarity necessitates initial axonal outgrowth in concomitance with the additionof new membrane to the axon´s plasmalemma. Axolemmal expansion occurs byexocytosis of plasmalemmal precursor vesicles primarily at the neuronal growth conemembrane. The multiprotein exocyst complex drives spatial location and specificity ofvesicle fusion at plasma membrane. However, the specific participation of its differentproteins on neuronal differentiation has not been fully established. In the presentwork we analyzed the role of Sec3, a prominent exocyst complex protein on neuronaldifferentiation. Using mice hippocampal primary cultures, we determined that Sec3 isexpressed in neurons at early stages prior to neuronal polarization. Furthermore, wedetermined that silencing of Sec3 in mice hippocampal neurons in culture precludedpo