Histone H1 is dispensable for Methylation-associated gene silencing in Ascobolus and essential for long life-span.

JOSÉ LUIS BARRA; L. RHOUNIM,; G. FAUGERON,; J.-L. ROSSIGNOL,

AMER SOC MICROBIOLOGY

Año: 2000 vol. 20 p. 61 - 61

b> Ascobolus immersus. The deduced peptide sequence presents the characteristic three-domain structure of metazoan linker histones, with a central globular region, an N-terminal tail, and a long positively charged C-terminal tail. By constructing an artificial duplication of this gene, named H1, it was possible to methylate and silence it by the MIP (methylation induced premeiotically) process. This resulted in the complete loss of the Ascobolus H1 histone. Mutant strains lacking H1 displayed normal methylation-associated gene silencing, underwent MIP, and showed the same methylation-associated chromat