Cloning and functional expression of secreted phospholipases A(2) from Bothrops diporus (Yarará Chica)

YUNES QUARTINO PJ; BARRA JL; FIDELIO GD

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2012 vol. 427 p. 321 - 321

font size="1"> Bothrops diporus is a very common viper in Argentina. At present, no complete sequence of secreted phospholipase A2 (sPLA2) from this snake has been reported. We have cloned two sPLA2 isoenzymes as well as a putative sPLA2-like myotoxin from venom gland. The two sPLA2 were expressed as inclusion bodies in Escherichia coli with an N-terminal tag of ubiquitin. After in vitro renaturation and cleavage step, using an ubiquitin specific peptidase, the recombinants exhibited sPLA2 activity when analyzed by means of Langmuir dilauroylphosphatidylcholine monolayers as substrate. Both enzymes have a similar surface pressure- activity profile when compared with non-recombinant purified isoforms. To our knowledge, this is the first time that analysis of op