Early phenotipic and genotypic alterations in submandibular gland oncogenesis in rats

M. BRUNOTTO,; A. MALBERTI,; M. ZÁRATE, A.; JOSÉ LUIS BARRA; O. CALDERÓN,; E. PIÑAS,; L. PLAVNIK,; M. CROSA,

Buenos Aires : Acta Odontologica Latinoamericana

Año: 2006 vol. 19 p. 13 - 13

p class="abstract">The present study evaluates the phenotypic and genotypic changes that take place during early oncogenesis. The submandibular glands of male rats were injected with a 0.5% solution of 9,10-dimethyl-1,2-benzanthracene (DMBA) in acetone. Gland samples were taken at 0, 7, 30 and 150 days post-injection and submitted to histological, biochemical, immunocytochemical and PCR evaluation. Histopathological analysis was performed on hematoxylin-eosin stained slides. Total protein content was assessed by Lowry´s method and the protein profile was analyzed by 12% SDS-PAGE. Bcl-2 was demonstrated by silver-enhanced gold immunolabeling. p53 immunolabeling was performed using the streptavidin-biotin system. All the treated animals developed carcinoma-like lesions at 30 and 150 days. Total protein concentration rose significantly (p < 0.05) above control values at 7, 30 and 150 days. The treated glands exhibited positive immunolabeling for p53 in the nuclei of neoplastic cells a