Induction of the formyl peptide receptor 2 in microglia by IFN-gamma and synergy with CD40 ligand.

CHEN K,; IRIBARREN P,; HUANG J,; ZHANG L,; GONG W,; CHO EH,; LOCKETT S,; DUNLOP NM,; WANG JM

JOURNAL OF IMMUNOLOGY

Año: 2007 vol. 178 p. 1759 - 1759

0022-1767

p class="abstract">Human formyl peptide receptor (FPR)-like 1 (FPRL1) and its mouse homologue mFPR2 are functional receptors for a variety of exogenous and host-derived chemotactic peptides, including amyloid beta 1-42 (Abeta(42)), a pathogenic factor in Alzheimer´s disease. Because mFPR2 in microglial cells is regulated by proinflammatory stimulants including TLR agonists, in this study we investigated the capacity of IFN-gamma and the CD40 ligand (CD40L) to affect the expression and function of mFPR2. We found that IFN-gamma, when used alone, induced mFPR2 mRNA expression in a mouse microglial cell line and primary microglial cells in association with increased cell migration in response to mFPR2 agonists, including Abeta(42). IFN-gamma also increased the endocytosis of Abeta(42) by microglial cells via mFPR2. The effect of IFN-gamma on mFPR2 expression in microglial cells was dependent on activation of MAPK and IkappaB-alpha. IFN-gamma additionally increased the expression of CD40