Involvement of peritoneal dendritic cells in the induction of autoimmune prostatitis.

CORREA S; RIERA CM; PABLO IRIBARREN

J Autoimmun

Elsevier

Año: 1997 vol. 10 p. 107 - 107

e have been working within a model of autoimmune prostatitis induced bythe intraperitoneal administration of saline extract of rat male accessoryglands (RAG) associated to liposomes. The intraperitoneal administration ofRAG-liposomes elicits both primary and secondary cellular autoimmuneresponses to RAG as well as organ-specific lesions. To evaluate the participationof dendritic cells (DC) in the induction of the autoimmune response, wepurified peritoneal DC (PDC) after a single injection of RAG-liposomes andwe characterized this population by morphology and phenotype. Based onadherence and morphologic criteria, we determined that PDC comprisedapproximately 1% of the total peritoneal cells. The ultrastructure of thedendritic cell enriched fraction was assessed by electron microscopy. By FACSanalysis, PDC showed a two to three-fold increase in expression of the IAmolecule compared to macrophages. They expressed low