IRIBARREN PABLO
Artículos
Título:
Granulocyte-macrophage colony-stimulating factor protects dendritic cells from liposome-encapsulated dichloromethylene diphosphosnate-induced apoptosis through a bcl-2-mediated pathway.
Autor/es:
RABINOVICH, G.; RIERA CM; PABLO IRIBARREN
Revista:
Eur J Immunol
Editorial:
Wiley
Referencias:
Año: 1999 vol. 29 p. 563 - 563
Resumen:
iposome-encapsulated dichloromethylene diphosphonate (L-MDP) has been used fordepleting cells of the monocyte-macrophage lineage. We have undertaken this study toinvestigate whether dendritic cells are susceptible to this liposome-encapsulated compound.Dendritic cells were cultured in the presence of L-MDP and further processed forapoptosis detection. The highly characteristic DNA cleavage into oligonucleosome-sizedfragments, incorporation of biotinylated dUTP into DNA strand breaks and the typical ultrastructuralfeatures of apoptosis were evident in dendritic cells exposed to the drug. Moreimportantly, we demonstrated that granulocyte-macrophage colony-stimulating factor protectsdendritic cells not only from apoptosis induced by the exogenous compound but alsofrom spontaneous apoptosis. Western blot analysis revealed that this protection was tightlycorrelated with the activation of a Bcl-2-mediated pathway. Regulation of the