IRIBARREN PABLO
Artículos
Título:
Co-expression of IL-18 strongly attenuates IL-12-induced systemic toxicity through a rapid induction of IL-10 without affecting its anti-tumor capacity
Autor/es:
RODRIGUEZ-GALAN, M. C.; REYNOLDS, D; COREA, SG; IRIBARREN, P; WATANABE, M; YOUNG, HA
Editorial:
AMER ASSOC IMMUNOLOGISTS
Referencias:
Año: 2009 p. 740 - 740
Resumen:
style> body { background: #FFFFFF; margin: 0px; padding: 0px; } IL-12 is an excellent candidate for the treatment of cancer due to its ability to drive strong anti-tumor responses. Recombinant IL-12 protein is currently used in cancer patients; however, systemic expression of rIL-12 presents disadvantages including cost and dose limitation due to its toxicity. In this study we used hydrodynamic shear of cDNA as a tool to achieve systemic expression of IL-12. We found that sustained but toxic levels of serum IL-12 could be generated in 6-7 week old B6 mice after a single injection of the cDNA. Unexpectedly, we observed that when IL-12 cDNA is co-injected with IL-18 cDNA, IL-12 anti-tumor activity was maintained but there was a significant attenuation of IL-12 toxicity, as evidenced by a greater survival index and a diminution of liv