Type I IFNs Are Required to Promote Central Nervous System Immune Surveillance through the Recruitment of Inflammatory Monocytes upon Systemic Inflammation

PERALTA RAMOS, JAVIER MARÍA; BUSSI, CLAUDIO; GAVIGLIO, EMILIA ANDREA; ARROYO, DANIELA SOLEDAD; BAEZ, NATALIA SOLEDAD; RODRIGUEZ-GALAN, MARIA CECILIA; IRIBARREN, PABLO

Frontiers in Immunology

Frontiers

Año: 2017 vol. 8

rain-resident microglia and peripheral migratory leukocytes play essential roles inshaping the immune response in the central nervous system. These cells activate andmigrate in response to chemokines produced during active immune responses andmay contribute to the progression of neuroinflammation. Herein, we addressed the participationof type I?II interferons in the response displayed by microglia and inflammatorymonocytes to comprehend the contribution of these cytokines in the establishment anddevelopment of a neuroinflammatory process. Following systemic lipopolysaccharide(LPS) challenge, we found glial reactivity and an active recruitment of CD45hi leukocytesclose to CD31+ vascular endothelial cells in circumventricular organs. Isolated CD11b+CD45hi Ly6Chi Ly6G−-primed inflammatory monocytes were able to induce T cell proliferation,unlike CD11b+ CD45lo microglia. Moreover, ex vivo re-stimulation with LPSexhibi