IRIBARREN PABLO
Congresos y reuniones científicas
Título:
DIFFERENTIAL REGULATION OF IGF-1 PRODUCTION IN MICROGLIAL CELLS STIMULATED WITH IL-4 OR IFN GAMMA
Autor/es:
GAVIGLIO, EA; PERALTA RAMOS, JM; ARROYO, DS; BUSSI, C; RODRIGUEZ-GALAN, MC; IRIBARREN, P
Lugar:
Los Cocos
Reunión:
Congreso; Reunion Anual de la Sociedad Argentina de Inmunología; 2013
Institución organizadora:
SAI
Resumen:
Insulin like growth factor-1 (IGF-1) is involved in the regulation of growth and tissue differentiation in various
organs, including the brain. This factor is synthesised by hepatocytes and released at blood flow or it is
produced by muscular and neural cells. In the brain, IGF-1 is one of the most important neurotrophic factors
involved in neural cell survival, proliferation and differentiation and it has been related to neuroprotective
functions.
Microglial cells (MC) are phagocytic myeloid cells located in the nervous parenchyma that have a key immune
role in the initiation, progression and resolution of neuroinflammation. When activated, they can perform
many diverse functions which may be either beneficial or harmful depending on the stimuli.
Therefore, the aim of this study was to determine in vitro if MC were capable of producing IGF-1 and if this
production was regulated by IFN gamma (type 1 cytokine) or IL-4 (type 2 cytokine).
First of all, we evaluated by real time PCR the effects of the stimulation of murine MC lines with IL-4 or INF
gamma, on the expression of the gene coding for IGF-1. IL-4 dose-dependently increased IGF-1 gene expression
from 6h to 48h after stimulation (p<0,0001). On the other hand, IFN gamma decreased IGF-1 gene expression in
MC and this response was time-dependent (p<0,001). Then we evaluated the production of IGF-1 by ELISA, and
we observed that IL-4 increased IGF-1 levels in MC at 24h (p<0,01) whereas IFN gamma induced a decrease in
the IGF-1 secretion (p<0,01).
Finally, we observed that IL-4 was able to modulate the IGF-1 gene expression on IFN gamma-stimulated MC.
We are performing additional experiments to evaluate the effects of supernatants from MC stimulated with IL-
4 or IFN gamma, on neuronal cell differentiation and survival.
Overall, these preliminary results suggest that type 1 and 2 cytokines are effective modulators of IGF-1
production by MC, which may be important in the regulation of neurodegenerative diseases.
organs, including the brain. This factor is synthesised by hepatocytes and released at blood flow or it is
produced by muscular and neural cells. In the brain, IGF-1 is one of the most important neurotrophic factors
involved in neural cell survival, proliferation and differentiation and it has been related to neuroprotective
functions.
Microglial cells (MC) are phagocytic myeloid cells located in the nervous parenchyma that have a key immune
role in the initiation, progression and resolution of neuroinflammation. When activated, they can perform
many diverse functions which may be either beneficial or harmful depending on the stimuli.
Therefore, the aim of this study was to determine in vitro if MC were capable of producing IGF-1 and if this
production was regulated by IFN gamma (type 1 cytokine) or IL-4 (type 2 cytokine).
First of all, we evaluated by real time PCR the effects of the stimulation of murine MC lines with IL-4 or INF
gamma, on the expression of the gene coding for IGF-1. IL-4 dose-dependently increased IGF-1 gene expression
from 6h to 48h after stimulation (p<0,0001). On the other hand, IFN gamma decreased IGF-1 gene expression in
MC and this response was time-dependent (p<0,001). Then we evaluated the production of IGF-1 by ELISA, and
we observed that IL-4 increased IGF-1 levels in MC at 24h (p<0,01) whereas IFN gamma induced a decrease in
the IGF-1 secretion (p<0,01).
Finally, we observed that IL-4 was able to modulate the IGF-1 gene expression on IFN gamma-stimulated MC.
We are performing additional experiments to evaluate the effects of supernatants from MC stimulated with IL-
4 or IFN gamma, on neuronal cell differentiation and survival.
Overall, these preliminary results suggest that type 1 and 2 cytokines are effective modulators of IGF-1
production by MC, which may be important in the regulation of neurodegenerative diseases.
