IRIBARREN PABLO
Congresos y reuniones científicas
Título:
Induction of autophagy in BV2 microglial cells modulates pro-inflammatory mediator levels and rescues both LPS and alpha-synuclein-induced neuronal cell death
Autor/es:
BUSSI, C; PERALTA RAMOS, JM; GAVIGLIO, EA; ARROYO, DS; GALLEA, JI; CELEJ, MS; IRIBARREN, P
Reunión:
Congreso; II French-Argentinean Immunology Meeting / SAI; 2015
Institución organizadora:
SAI
Resumen:
Abstract: Autophagy is a fundamental cellular homeostatic mechanism, whereby cells autodigest parts of their cytoplasm for removal or turnover. Despite the increasing reports studying the effects of autophagy in the CNS, slightly emphasis is placed on microglial cells.
The aim of this study was to evaluate the effects of autophagy on the production of pro-inflammatory mediators by BV2 microglial cells, and on neuronal viability in a co-culture model. Autophagy was induced before or after TLR stimulation by rapamycin or trehalose and blocked by using 3-Methyladenine. Autophagy induction in BV2 cells before LPS or alpha-synuclein stimulation downregulated IL1b, IL-6, TNFa and nitric oxide production.
Furthermore, we observed in BV2/N2A co-cultures stimulated with LPS or alpha-synuclein fibers that induction of autophagy in microglial cells rescued LPS and alpha-synuclein-induced neuronal cell death.
These results suggest that modulation of microglial cells by autophagy could be an important strategy in the context of neurodegenerative diseases.